Gene name |
SPBC19C7.09c |
Gene ID |
33/D09 |
Gene synonyms/obsolete |
uve1; uvde |
Gene product |
UV-endonuclease;
involved in DNA repair; involved in nucleotide-excision
repair; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1800 |
ORF length (spliced) |
|
Entry clone length |
1800 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
531T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC19C7.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTAGGCTATTGAAACG |
Rev primer name |
SPBC19C7.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTCATCCTCTTCTACT |
Amino acid length |
599 |
Molecular weight |
68.8 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
584 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSDSVKARLVL/LLPLCQELNI |
Localization (YFP) |
nucleus;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |