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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19C7.09c
Gene ID 33/D09
Gene synonyms/obsolete uve1; uvde
Gene product UV-endonuclease; involved in DNA repair; involved in nucleotide-excision repair; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1800
ORF length (spliced)
Entry clone length 1800
No. of intron 0
Sequence status Finished
Sequence results 531T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC19C7.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTAGGCTATTGAAACG
Rev primer name SPBC19C7.09.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTTCATCCTCTTCTACT
Amino acid length 599
Molecular weight 68.8
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 584
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSDSVKARLVL/LLPLCQELNI
Localization (YFP) nucleus; mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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