| Gene name |
SPBC543.04 |
| Gene ID |
33/E02 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; UPF0171 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1823 |
| ORF length (spliced) |
1758 |
| Entry clone length |
1823 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC543.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTACGCTTAACTCCTCG |
| Rev primer name |
SPBC543.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCAAGAATGCCATCGAAGG |
| Amino acid length |
585 |
| Molecular weight |
66.8 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPSLSTFLAI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
DeltaVision |