Gene name |
SPBP23A10.01c |
Gene ID |
33/H06 |
Gene synonyms/obsolete |
pmc1;
SPBC1A4.10c |
Gene product |
RNA polymerase II
holoenzyme component; mediator complex subunit; involved in
regulating RNA polymerase II activity |
Entry clone |
Cloned |
ORF length (unspliced) |
2640 |
ORF length (spliced) |
|
Entry clone length |
2640 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBP23A10.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACCGCCTGCGATTCC |
Rev primer name |
SPBP23A10.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTTTCGAGAGATCGCT |
Amino acid length |
879 |
Molecular weight |
100.8 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTAILKRILAI |
Localization (YFP) |
nuclear dots;
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol; nuclear dots |
Microscope used for
observation |
DeltaVision |