| Gene name |
SPBC4F6.06 |
| Gene ID |
33/H07 |
| Gene synonyms/obsolete |
kin1 |
| Gene product |
serine/threonine
protein kinase; involved in cell polarity (implicated);
involved in cellular morphogenesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2676 |
| ORF length (spliced) |
|
| Entry clone length |
2676 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
2211T:C /
2244T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC4F6.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGTACCGTACCAATAA |
| Rev primer name |
SPBC4F6.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAATTTTAATTCGTTAAGA |
| Amino acid length |
891 |
| Molecular weight |
98.7 |
| Isoelectric point (calc.) |
10.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIRVMGMLDI/LASRILNELKL |
| Localization (YFP) |
cytoplasmic dots at
cell tip with filamentous structures; periphery at site of
septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |