| Gene name |
SPBC31E1.02c |
| Gene ID |
33/H08 |
| Gene synonyms/obsolete |
pgak2 |
| Gene product |
P-type calcium ATPase;
P2 type |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2700 |
| ORF length (spliced) |
|
| Entry clone length |
2700 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
798A:G / 1273T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC31E1.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGTTCAATATGATGC |
| Rev primer name |
SPBC31E1.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACATTCCTTAGCAGATAA |
| Amino acid length |
899 |
| Molecular weight |
98.3 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
7 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKQFVKDPLIL/LPIIVTVTLAL/LSTSVAALSL/LNAMQILWI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |