| Gene name |
SPAC20G8.06 |
| Gene ID |
34/C04 |
| Gene synonyms/obsolete |
|
| Gene product |
CCR4-Not complex |
| Entry clone |
Cloned; Mixture |
| ORF length (unspliced) |
6303 |
| ORF length (spliced) |
|
| Entry clone length |
6303 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1632A:G /
6283G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC20G8.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAGCCAAGAACAACA |
| Rev primer name |
SPAC20G8.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACTTGACCCATCAGTTCT |
| Amino acid length |
2100 |
| Molecular weight |
237.1 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDEKVSAELLL/LFAFLRSLWL/LILGIPSLQL/LQVSFKDLLL/LNLKFEIELLL/LHLNLPSVLKL/LYENFDRLSL/LFRKIIALII/LMSFLKFLDL/LSAFPSDLHL/LEKFASALDL |
| Localization (YFP) |
cytosol=nucleus;
cytosol |
| Comments for localization |
too dark to
photo |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |