| Gene name |
SPAC9.03c |
| Gene ID |
34/C06 |
| Gene synonyms/obsolete |
brr2 |
| Gene product |
U5 snRNP-specific
protein; DEAD/DEAH box helicase; RNA helicase; helicase
C-terminal domain; AAA family ATPase; Sec63 domain; involved
in mRNA splicing; complexed with Cdc5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
6531 |
| ORF length (spliced) |
|
| Entry clone length |
6531 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
945T:C / 4316A:G /
4446C:T / 5630A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC9.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAGTGCACATCCTAA |
| Rev primer name |
SPAC9.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCCCCGTCTTCCATTTCT |
| Amino acid length |
2176 |
| Molecular weight |
248.8 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLMNQQLPI |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |