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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13C5.05c
Gene ID 34/E04
Gene synonyms/obsolete
Gene product N-acetylglucosamine-phosphate mutase (putative)
Entry clone Cloned
ORF length (unspliced) 1642
ORF length (spliced) 1557
Entry clone length 1642
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC13C5.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACCAAGAACAAAAAATA
Rev primer name SPAC13C5.05.Rv
Rev primer SEQ AGAAAGCTGGGTATAATACTAGCTGACAAACT
Amino acid length 518
Molecular weight 56.3
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LCEKVCQLVL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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