Gene name |
SPBC83.03c |
Gene ID |
34/F08 |
Gene synonyms/obsolete |
|
Gene product |
sequence orphan;
hypothetical protein; serine-rich protein |
Entry clone |
Cloned# |
ORF length (unspliced) |
1650 |
ORF length (spliced) |
|
Entry clone length |
1650 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC83.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGAAAGGAATCAAAAA |
Rev primer name |
SPBC83.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTCGTTTTTATGCTCT |
Amino acid length |
549 |
Molecular weight |
63 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIDFSQVLQL |
Localization (YFP) |
SPB?; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |