Gene name |
SPAC823.03 |
Gene ID |
34/F09 |
Gene synonyms/obsolete |
SPAC1E11.03 |
Gene product |
serine/threonine
protein kinase |
Entry clone |
Cloned |
ORF length (unspliced) |
1652 |
ORF length (spliced) |
1605 |
Entry clone length |
1652 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC823.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTCGGATTCGCCCAT |
Rev primer name |
SPAC823.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGAAAAATTCATCTACATTT |
Amino acid length |
534 |
Molecular weight |
61.4 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCLVFELLSI/LGCILAELFL |
Localization (YFP) |
SPB?; cytoplasmic dots
at cell tip; cytosol |
Comments for localization |
cytoplasmic dots near
nucleus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal,
DeltaVision |