| Gene name |
SPBC577.07 |
| Gene ID |
34/G07 |
| Gene synonyms/obsolete |
ubp10 |
| Gene product |
ubiquitin C-terminal
hydrolase activity; zinc finger protein; zf-UBP type; putative
snRNP assembly factor; involved in mRNA spcing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1658 |
| ORF length (spliced) |
1509 |
| Entry clone length |
1658 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
31T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC577.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGAAAATAAGGATAA |
| Rev primer name |
SPBC577.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGATGACCTTTCCCAAAGC |
| Amino acid length |
502 |
| Molecular weight |
58.8 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |