| Gene name |
SPCC1322.13 |
| Gene ID |
34/G10 |
| Gene synonyms/obsolete |
ade6; min1 |
| Gene product |
phosphoribosylaminoimidazole carboxylase; AIR
carboxylase; involved in purine biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1659 |
| ORF length (spliced) |
|
| Entry clone length |
1659 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
343A:G / 438A:T /
1174A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1322.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCGAAAAACAGGTTGT |
| Rev primer name |
SPCC1322.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCAGAATAATTTTTCCAA |
| Amino acid length |
552 |
| Molecular weight |
60 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
except nucleolus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |