Gene name |
SPAC20G8.03 |
Gene ID |
35/A05 |
Gene synonyms/obsolete |
itr2 |
Gene product |
MFS myo-inositol
transporter; similar to Sp itr1 |
Entry clone |
Cloned |
ORF length (unspliced) |
1674 |
ORF length (spliced) |
|
Entry clone length |
1674 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC20G8.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTTCAACAATATTCC |
Rev primer name |
SPAC20G8.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCTTCATCTATTCTTCCT |
Amino acid length |
557 |
Molecular weight |
61.1 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
|
No. of transmembrane domain |
10 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |