| Gene name |
SPBC17D1.02 |
| Gene ID |
35/B04 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in
peptidyl-diphthamide biosynthesis from
peptidyl-histidine |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1678 |
| ORF length (spliced) |
1512 |
| Entry clone length |
1678 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
176T:deletion /
627A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC17D1.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTTGAATTATCAGC |
| Rev primer name |
SPBC17D1.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTACCAAAATCACGTTGA |
| Amino acid length |
503 |
| Molecular weight |
55.6 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYVFGRLPI/LHKLEKCLTI/LQDMTLLYI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |