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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23D3.12
Gene ID 35/B05
Gene synonyms/obsolete
Gene product inorganic phosphate transporter; similar to Sp SPBC8E4.01c (paralog)
Entry clone Cloned
ORF length (unspliced) 1680
ORF length (spliced)
Entry clone length 1680
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23D3.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATCGCTTAAATCCTTT
Rev primer name SPAC23D3.12.Rv
Rev primer SEQ AGAAAGCTGGGTAGAAAATTCCGTTGAAATAC
Amino acid length 559
Molecular weight 62.3
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LALVPALLVL
Localization (YFP) Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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