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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22A12.01c
Gene ID 35/B10
Gene synonyms/obsolete SPAC56F8.17c
Gene product damaged DNA binding activity; involved in DNA repair and double-strand break repair
Entry clone Cloned
ORF length (unspliced) 1683
ORF length (spliced)
Entry clone length 1683
No. of intron 0
Sequence status Finished
Sequence results 1363T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22A12.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTAAACGAAAGTCATC
Rev primer name SPAC22A12.01.Rv
Rev primer SEQ AGAAAGCTGGGTACCAATCCACATTTTCCAAT
Amino acid length 560
Molecular weight 63
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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