| Gene name |
SPAC22A12.09c |
| Gene ID |
35/C02 |
| Gene synonyms/obsolete |
sap114 |
| Gene product |
involved in mRNA
splicing; Surp domains; complexed with Cdc5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1686 |
| ORF length (spliced) |
1446 |
| Entry clone length |
1686 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
184T:C / 293T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22A12.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTCTTTAATGGAATT |
| Rev primer name |
SPAC22A12.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCGGCGTTTATTTGTGGGA |
| Amino acid length |
481 |
| Molecular weight |
54.4 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPSISSLDL |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |