Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC16C4.07
Gene ID 35/C03
Gene synonyms/obsolete scw1
Gene product RNA-binding protein; involved in septation and regulation of septum formation; regulates cell wall structure; rrm RNA recognition motif
Entry clone Cloned
ORF length (unspliced) 1686
ORF length (spliced)
Entry clone length 1686
No. of intron 0
Sequence status Finished
Sequence results 208G:A / 531C:T / 786G:C / 1062A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC16C4.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTGTGGGATCACCGAG
Rev primer name SPCC16C4.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTTGCCATACATTAGATTA
Amino acid length 561
Molecular weight 60.3
Isoelectric point (calc.) 7.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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