| Gene name |
SPAC6F12.02 |
| Gene ID |
35/D09 |
| Gene synonyms/obsolete |
rst2 |
| Gene product |
transcriptional
regulator; zinc finger protein; zf-C2H2 type; regulator of
sexual development; regulates ste11; regulator of
gluconeogenesis; phosphorylated by protein kinase A |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1704 |
| ORF length (spliced) |
|
| Entry clone length |
1704 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
439T:C / 453T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6F12.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGCGTGAGTCTTTAGC |
| Rev primer name |
SPAC6F12.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAATGAGGGCGGTTGATTC |
| Amino acid length |
567 |
| Molecular weight |
62.5 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
| Microscope used for
observation |
DeltaVision |