| Gene name |
SPAC343.04c |
| Gene ID |
35/D08 |
| Gene synonyms/obsolete |
|
| Gene product |
WD repeat
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1702 |
| ORF length (spliced) |
1524 |
| Entry clone length |
1702 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1269A:G /
1644C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC343.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTTGGATGAAAAATT |
| Rev primer name |
SPAC343.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGTCGACGTGGATTATCT |
| Amino acid length |
507 |
| Molecular weight |
58.4 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?;
nucleus>cytosol |
| Comments for localization |
cytoplasmic dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |