| Gene name |
SPAC4G8.05 |
| Gene ID |
35/D07 |
| Gene synonyms/obsolete |
|
| Gene product |
serine/threonine
protein kinase; similar to Sp SPBC1861.09 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1701 |
| ORF length (spliced) |
|
| Entry clone length |
1701 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1158A:G /
1309A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4G8.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGAACTTCATGATGG |
| Rev primer name |
SPAC4G8.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCCCACTCATGATGCACA |
| Amino acid length |
566 |
| Molecular weight |
63.4 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
124 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery, especially
at cell tip and site of septum formation;
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to: periphery;
nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |