| Gene name |
SPAC6F12.05c |
| Gene ID |
35/E08 |
| Gene synonyms/obsolete |
tnr3 |
| Gene product |
thiamine
pyrophosphokinase (thiamine kinase); regulator of thiamine
metabolism; regulator of phosphate metabolism, mating, and
growth; Nudix family hydrolase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1710 |
| ORF length (spliced) |
|
| Entry clone length |
1710 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1380T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6F12.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAATGTCGTCTATCAC |
| Rev primer name |
SPAC6F12.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAGACGTGTTTCCATTGTC |
| Amino acid length |
569 |
| Molecular weight |
64.2 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNQVLHELEL |
| Localization (YFP) |
cytosol; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |