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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F3.08
Gene ID 35/F11
Gene synonyms/obsolete sut1
Gene product alpha-glucoside transporter; function overlapping with?; glycoside-pentoside-hexuronide cation symporter family; major facilitator superfamily; involved in maltose uptake; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1728
ORF length (spliced) 1662
Entry clone length 1728
No. of intron 1
Sequence status Finished
Sequence results 294A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2F3.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGTAGATGAAAATCA
Rev primer name SPAC2F3.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGTTGATGGGCAAAAGA
Amino acid length 553
Molecular weight 61.7
Isoelectric point (calc.) 6.3
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LGYLLGYLPL
Localization (YFP) Golgi; vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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