| Gene name |
SPAC29A4.01c |
| Gene ID |
35/F12 |
| Gene synonyms/obsolete |
SPAC26F1.14c |
| Gene product |
flavoprotein; similar
to human mitochondrial apoptosis-inducing factor; flavoprotein
inducing chromatin condensation and DNA fragmentation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1728 |
| ORF length (spliced) |
|
| Entry clone length |
1728 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
254G:A / 319T:C /
662A:G / 783T:C / 812T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC29A4.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAGTAGTTCAGGCTT |
| Rev primer name |
SPAC29A4.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAAAGGAATGGACAACACA |
| Amino acid length |
575 |
| Molecular weight |
62.1 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLHDISKLAL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |