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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC16A11.08
Gene ID 36/C11
Gene synonyms/obsolete
Gene product sorting nexin; PX domain; involved in intracellular protein transport; phosphoinositide binding
Entry clone Cloned
ORF length (unspliced) 1782
ORF length (spliced) 1605
Entry clone length 1782
No. of intron 2
Sequence status Finished
Sequence results 893C:T / 921A:G / 960T:C / 1135T:C / 1404A:G / 1496A:G / 1720A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC16A11.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCACAAGACGATTCTTA
Rev primer name SPCC16A11.08.Rv
Rev primer SEQ AGAAAGCTGGGTATATGTCCTTCGATAAATTT
Amino acid length 534
Molecular weight 60.1
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots; periphery
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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