Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1773.16c
Gene ID 36/D02
Gene synonyms/obsolete
Gene product transcriptional regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain
Entry clone Cloned
ORF length (unspliced) 1788
ORF length (spliced)
Entry clone length 1788
No. of intron 0
Sequence status Finished
Sequence results 913A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1773.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAAGGATCAGAAATGC
Rev primer name SPBC1773.16.Rv
Rev primer SEQ AGAAAGCTGGGTAAATTGAAAAACTTTCGCTC
Amino acid length 595
Molecular weight 68.8
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEKIQALVI
Localization (YFP) nucleus; bright nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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