| Gene name |
SPAC24C9.08 |
| Gene ID |
36/D03 |
| Gene synonyms/obsolete |
|
| Gene product |
gly x carboxypeptidase
activity; peptidase family M20 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1791 |
| ORF length (spliced) |
|
| Entry clone length |
1791 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
753T:G / 1313G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC24C9.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCACTTCTAACGACCC |
| Rev primer name |
SPAC24C9.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACGCTTCTTCACCGGAATCA |
| Amino acid length |
596 |
| Molecular weight |
66.6 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVRFQELLRI/LEALEILAI |
| Localization (YFP) |
vacuole |
| Comments for localization |
too dark to
photo |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |