Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3B8.09
Gene ID 36/D04
Gene synonyms/obsolete
Gene product ribonucleoprotein (RNP) complex; small subunit (SSU) processome component; involved in rRNA processing; involved in establishment and/or maintenance of chromatin architecture; involved in telomere repression
Entry clone Cloned
ORF length (unspliced) 1794
ORF length (spliced)
Entry clone length 1794
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3B8.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAGAAAGAAGTCGAG
Rev primer name SPBC3B8.09.Rv
Rev primer SEQ AGAAAGCTGGGTATTGAAGTTTAATACTTTTA
Amino acid length 597
Molecular weight 68
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 149
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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