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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC2F12.14c
Gene ID 37/A02
Gene synonyms/obsolete gua1
Gene product IMP dehydrogenase activity; involved in purine biosynthesis; involved in guanine biosynthesis; CBS domain protein
Entry clone Cloned
ORF length (unspliced) 1907
ORF length (spliced) 1575
Entry clone length 1907
No. of intron 1
Sequence status Finished
Sequence results 1056A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC2F12.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGCCTTTAAGCCTTA
Rev primer name SPBC2F12.14.Rv
Rev primer SEQ AGAAAGCTGGGTAGTAAAGACGCTTTTCGTAT
Amino acid length 524
Molecular weight 57
Isoelectric point (calc.) 6.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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