Gene name |
SPAC1556.02c |
Gene ID |
37/C02 |
Gene synonyms/obsolete |
sdh1 |
Gene product |
succinate
dehydrogenase (ubiquinone); FAD binding domain; flavoprotein
subunit; involved in tricarboxylic acid cycle; involved in
mitochondrial electron transport, succinate to ubiquinone;
involved in succinate metabolism; succinate dehydrogenase
(ubiquinone) activity |
Entry clone |
Cloned |
ORF length (unspliced) |
2047 |
ORF length (spliced) |
1926 |
Entry clone length |
2047 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
268A:T / 313T:C /
437A:G / 446A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1556.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTCAGATTTCGAAAAGT |
Rev primer name |
SPAC1556.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATAAACACGTTTGAAAGGA |
Amino acid length |
641 |
Molecular weight |
70.4 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
aggregates |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |