| Gene name |
SPBC3B8.12 |
| Gene ID |
37/C05 |
| Gene synonyms/obsolete |
SPBC11C11.11c |
| Gene product |
DEAD/DEAH box
helicase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2050 |
| ORF length (spliced) |
1821 |
| Entry clone length |
2050 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
1932T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3B8.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTACTAAACGCATGTTG |
| Rev primer name |
SPBC3B8.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACCCTTTAATTCCTTCAAT |
| Amino acid length |
606 |
| Molecular weight |
68.9 |
| Isoelectric point (calc.) |
10 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTQMIGRGLRL/LSFLQKVLII |
| Localization (YFP) |
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |