| Gene name |
SPAC1F7.03 |
| Gene ID |
37/D12 |
| Gene synonyms/obsolete |
|
| Gene product |
TRP-like ion channel;
essential; DUF907; conserved fungal protein; 9 predicted
transmembrane helices; predicted N-terminal signal
sequence |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2133 |
| ORF length (spliced) |
|
| Entry clone length |
2133 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1495T:C /
1715T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1F7.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGGCTTTGGAGAAGCCC |
| Rev primer name |
SPAC1F7.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGAAAAGCATTGTTAGGT |
| Amino acid length |
710 |
| Molecular weight |
79.9 |
| Isoelectric point (calc.) |
8.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
9 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNAITMLLLI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |