| Gene name |
SPAP7G5.03 |
| Gene ID |
37/F02 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved fungal
protein; transmembrane helices |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2152 |
| ORF length (spliced) |
2112 |
| Entry clone length |
2152 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1390A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAP7G5.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGTCTTCATATCTTAG |
| Rev primer name |
SPAP7G5.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATATCTATCCTGTTTTTCT |
| Amino acid length |
703 |
| Molecular weight |
78.7 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
5 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLIIIAGLFL/LKYFISALPL/LFFLLWLLI/LSSWLKSLFL/LISLSSDLAI |
| Localization (YFP) |
periphery; cytoplasmic
dots |
| Comments for localization |
ER? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica;
DeltaVision |