| Gene name |
SPAC1B3.10c |
| Gene ID |
37/F03 |
| Gene synonyms/obsolete |
|
| Gene product |
SEL1 repeat protein;
possibly extracellular by similarity to C. elegans sel-1; no
apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2152 |
| ORF length (spliced) |
2043 |
| Entry clone length |
2152 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
215A:G / 2020A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1B3.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGGTTTCGGTTCTGAC |
| Rev primer name |
SPAC1B3.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAATTAGCAGCTTTTATG |
| Amino acid length |
680 |
| Molecular weight |
78.7 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSIAFFLLSL/LMFEVAKLRL/LYIKLLKLLRL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |