Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.29
Gene ID 37/F07
Gene synonyms/obsolete
Gene product para-aminobenzoate synthase; involved in folate biosynthesis; involved in para-aminobenzoic acid metabolism
Entry clone Cloned
ORF length (unspliced) 2157
ORF length (spliced)
Entry clone length 2157
No. of intron 0
Sequence status Finished
Sequence results 1633A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP8B7.29.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTGAAATTTCAAACCG
Rev primer name SPBP8B7.29.Rv
Rev primer SEQ AGAAAGCTGGGTATTTACATGATCTTTTTTTA
Amino acid length 718
Molecular weight 80.5
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNRIWQLNI/LKIFKNFLSL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.