| Gene name |
SPAC890.03 |
| Gene ID |
38/B10 |
| Gene synonyms/obsolete |
|
| Gene product |
serine/threonine
protein kinase |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
2301 |
| ORF length (spliced) |
2019 |
| Entry clone length |
2301 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC890.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAAAAATGAAAAGGC |
| Rev primer name |
SPAC890.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAGCAAGTCTGTGAAATGT |
| Amino acid length |
672 |
| Molecular weight |
75.8 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGIIMYALLI |
| Localization (YFP) |
periphery at cell tip
and site of septum formation; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |