| Gene name |
SPAC4F10.09c |
| Gene ID |
38/E03 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in ribosome
biogenesis and assembly; CAATT-binding factor homolog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2583 |
| ORF length (spliced) |
|
| Entry clone length |
2583 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4F10.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAACGTTGTACCCCAA |
| Rev primer name |
SPAC4F10.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTCGAGTAAATGAGCA |
| Amino acid length |
860 |
| Molecular weight |
97.9 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
834 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNLLYKSLII/LFHVMHQLVI |
| Localization (YFP) |
nucleolus>>nucleus |
| Comments for localization |
one nuclear dot by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |