| Gene name |
SPAC24H6.13 |
| Gene ID |
38/F07 |
| Gene synonyms/obsolete |
|
| Gene product |
eukaryotic conserved
protein; Sc homolog RSN1 is involved in tunicamycin
sensitivity; DUF221 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2616 |
| ORF length (spliced) |
|
| Entry clone length |
2616 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
389T:C / 2116A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC24H6.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCGACAGCAGCAGTTC |
| Rev primer name |
SPAC24H6.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTCCTGATCGCCTCATCG |
| Amino acid length |
871 |
| Molecular weight |
98.4 |
| Isoelectric point (calc.) |
9.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
11 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
247/222 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFTFGALCI/LLQIVTLLL/LFQVFVGLYL/LTGFDRVLQL |
| Localization (YFP) |
Golgi; periphery;
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |