Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1039.05c
Gene ID 38/G08
Gene synonyms/obsolete
Gene product fungal conserved protein; zinc finger protein; zf-C2H2 type; similar to Sp zas1 (paralog)
Entry clone Cloned
ORF length (unspliced) 2651
ORF length (spliced) 2346
Entry clone length 2651
No. of intron 5
Sequence status Finished
Sequence results 106A:G / 693A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1039.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACCAAGGCGAAAAAAAG
Rev primer name SPAC1039.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGCTGTACTAATTTAGAC
Amino acid length 781
Molecular weight 89
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus; spindle microtubules
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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