| Gene name |
SPBC16C6.11 |
| Gene ID |
39/G09 |
| Gene synonyms/obsolete |
rpl3201; rpl32-1 |
| Gene product |
60S ribosomal protein
L32 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
384 |
| ORF length (spliced) |
|
| Entry clone length |
384 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16C6.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGCAATCAACATTGT |
| Rev primer name |
SPBC16C6.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTTGAGAACGAACTTTA |
| Amino acid length |
127 |
| Molecular weight |
14.4 |
| Isoelectric point (calc.) |
11.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol;
nucleolus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol, partially; nucleolus |
| Microscope used for
observation |
Leica |