Gene name |
SPBC337.14 |
Gene ID |
39/H10 |
Gene synonyms/obsolete |
rpb4 |
Gene product |
DNA-directed RNA
polymerase subunit DNA-directed RNA polymerase II (subunit);
essential; involved in transcription from Pol II promoter;
DNA-directed RNA polymerase activity (function of complex);
interacts physically with Rpb7p |
Entry clone |
Cloned |
ORF length (unspliced) |
565 |
ORF length (spliced) |
408 |
Entry clone length |
565 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
66T:C / 80A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC337.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCGAGGGCTATTTTTGA |
Rev primer name |
SPBC337.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTTGAAATTTACGCAAA |
Amino acid length |
135 |
Molecular weight |
15.3 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |