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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC23G7.05
Gene ID 39/H09
Gene synonyms/obsolete psu1; sui1
Gene product protein translation factor Sui1; translation factor
Entry clone Cloned
ORF length (unspliced) 545
ORF length (spliced) 330
Entry clone length 545
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC23G7.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGCTATTCAAAACTT
Rev primer name SPBC23G7.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGAAACCATGAATCTTGATG
Amino acid length 109
Molecular weight 12.4
Isoelectric point (calc.) 9.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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