| Gene name |
SPBC18H10.03 |
| Gene ID |
40/B07 |
| Gene synonyms/obsolete |
tif35 |
| Gene product |
translation initiation
factor; eukaryotic translation initiation factor 3 complex;
RNA-binding protein; rrm RNA recognition motif |
| Entry clone |
Cloned |
| ORF length (unspliced) |
890 |
| ORF length (spliced) |
849 |
| Entry clone length |
890 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC18H10.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTCATCGTATGTAAT |
| Rev primer name |
SPBC18H10.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTCGAGGCTTGGAGAAT |
| Amino acid length |
282 |
| Molecular weight |
31.4 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear dots;
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |