| Gene name |
SPAC1556.03 |
| Gene ID |
40/B08 |
| Gene synonyms/obsolete |
azr1 |
| Gene product |
serine/threonine
phosphatase family 2C (SMART); overexpression confers
resistance to 5-azacytidine (nucleoside analogue) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
917 |
| ORF length (spliced) |
867 |
| Entry clone length |
917 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1556.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGTTACTCGTCTTTA |
| Rev primer name |
SPAC1556.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACATATAAATTATTTTATCG |
| Amino acid length |
288 |
| Molecular weight |
31.9 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
32 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQFNMPYQLAI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |