| Gene name |
SPBC36.05c |
| Gene ID |
40/G04 |
| Gene synonyms/obsolete |
clr6 |
| Gene product |
transcriptional
regulator; cryptic loci regulator; histone deacetylase
activityj; NAD-independent histone deacetylase activity;
RPD3-like (class I); involved in transcriptional regulation;
involved in chromatin silencing; involved in chromatin
assembly/disassembly; essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1850 |
| ORF length (spliced) |
1218 |
| Entry clone length |
1850 |
| No. of intron |
13 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC36.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGTTCGGGAAGAAAAA |
| Rev primer name |
SPBC36.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACGCGCTCATCCATAATC |
| Amino acid length |
405 |
| Molecular weight |
46.1 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |