Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC4C3.12
Gene ID 40/G08
Gene synonyms/obsolete sep1; SPBC4C3.05b
Gene product hnf-3/fork head transcription factor homologue.
Entry clone Cloned
ORF length (unspliced) 1992
ORF length (spliced)
Entry clone length 1992
No. of intron 0
Sequence status Finished
Sequence results 45A:G / 1987T:deletion
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC4C3.05b.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATTTTAATTCTACTAA
Rev primer name SPBC4C3.05b.Rv
Rev primer SEQ AGAAAGCTGGGTAGAATAGTGTTGAAGTTTGA
Amino acid length 663
Molecular weight 73
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTYSVPPLPI
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation DeltaVision

Image information
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