| Gene name |
SPCC188.08c |
| Gene ID |
41/A06 |
| Gene synonyms/obsolete |
ubp5; ubp22 |
| Gene product |
ubiquitin C-terminal
hydrolase activity; peptidase family C19; similar to Sp ubp21
(paralog); double mutant ubp21 delta/ubp22 delta lethal; MATH
domain (SMART) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3457 |
| ORF length (spliced) |
3327 |
| Entry clone length |
3457 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
2337C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC188.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATTGAAGTTTTTGTT |
| Rev primer name |
SPCC188.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTTCATCTGAATAGCA |
| Amino acid length |
1108 |
| Molecular weight |
128.6 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCIELGQKLGI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol |
| Microscope used for
observation |
Leica |