| Gene name |
SPBC11C11.04c |
| Gene ID |
41/A08 |
| Gene synonyms/obsolete |
alp1 |
| Gene product |
tubulin-folding
cofactor d |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3581 |
| ORF length (spliced) |
3324 |
| Entry clone length |
3581 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
2025A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC11C11.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGAAGAAGAATCTTT |
| Rev primer name |
SPBC11C11.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCGATCAGCTATCAGTTTA |
| Amino acid length |
1107 |
| Molecular weight |
126.5 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFSLSSFLKI/LASIIGGFLSI/LLEIPNLRL |
| Localization (YFP) |
nuclear dots; spindle
microtubules; nucleus>>cytosol; periphery at site of
septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |