Gene name |
SPBC21.06c |
Gene ID |
41/A10 |
Gene synonyms/obsolete |
cdc7 |
Gene product |
serine/threonine
protein kinase; SIN component; involved in septation
(required); involved in cytokinesis (required);
essential |
Entry clone |
Cloned |
ORF length (unspliced) |
3650 |
ORF length (spliced) |
3189 |
Entry clone length |
3650 |
No. of intron |
10 |
Sequence status |
Finished |
Sequence results |
3636C:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC21.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCACAATATCCAAGCATC |
Rev primer name |
SPBC21.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTGCGTTAATGGCTGCTTT |
Amino acid length |
1062 |
Molecular weight |
119.2 |
Isoelectric point (calc.) |
7.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRRILRILLYL/LSMVPSSLSL |
Localization (YFP) |
nuclear dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |