Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP22H7.08
Gene ID 41/C07
Gene synonyms/obsolete rps1002; rps10-2; rps10B; pi023; SPACTOKYO_453.11
Gene product 40S ribosomal protein S10
Entry clone Cloned
ORF length (unspliced) 492
ORF length (spliced) 444
Entry clone length 492
No. of intron 1
Sequence status Finished
Sequence results 99G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP22H7.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTATTCCCAAGGAGAA
Rev primer name SPBP22H7.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGGCAGCAACAGGGCGACCG
Amino acid length 147
Molecular weight 16.4
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 2 images) See all images

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